Lp-PLA2 Activity Assay Kit
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Lp-PLA2 Activity Assay Kit is used for the quantitative determination of Lp-PLA2 enzyme activity in human serum and plasma samples.
Lp-PLA2, also known as platelet-activating factor acetylhydrolase (PAF-AH), is a phospholipase A2 enzyme produced by inflammatory cells. Lp-PLA2 is involved in the development of atherosclerosis and serves as a specific marker of cardiac disease. Studies found that Lp-PLA2 levels are positively correlated with increased risk of coronary disease, stroke, and mortality. Lp-PLA2 Activity Assay is an enzymatic assay. In the assay reaction, sample Lp-PLA2 hydrolyses acetyl group at the sn-2 position of the phospholipid substrate, 1- myristoyl-2-(4-nitrophenylsuccinyl)-sn-glycero-3-phosphocholine (MNP), resulting in a release of a colorful 4-nitrophenyl group, which can be monitored spectrophotometrically at 405-410 nm. Absorbance increase is proportional to the activity of Lp-PLA2, allowing the instrument to calculate the activity of a sample using the Lp-PLA2 Activity Calibrator generated curve. Reagents are Research Use Only. |
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Pricing available per request.
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