Vanillylmandelic Acid (VMA) Assay Kit
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【Intended Use】
Vanillylmandelic Acid (VMA) Assay is a quantitative homogeneous enzyme immunoassay for measuring VMA in human urine. VMA is an end-stage metabolite of the catecholamines: epinephrine, and norepinephrine. Catecholamines are secreted by chromaffin cells of the adrenal medulla and the postganglionic fibers of the sympathetic nervous system. Urinary VMA is elevated in patients with catecholamine secreting tumors including pheochromocytoma and neuroblastoma.VMA levels in urine are also related to adrenal medulla hyperplasia (AMH). Research shows that medulla hyperplasia, hypertension, nocturnal hypoxemia and congestive heart failure may lead to elevated Vanillylmandelic acid in patients’ urine. 【Assay Principle】 Our VMA assay is a competitive homogeneous enzyme immunoassay, where antibody-bound and free analytes are not separated. The result is based on the competition for specific antibody binding sites between the free VMA compound in the sample and VMA conjugated to Glucose-6-Phosphate Dehydrogenase (G6PDH) enzyme. The more free VMA is present in a sample, the less antibody is available to bind enzyme-VMA conjugate, which speeds up conversion of NAD+ to NADH. Change in NADH concentration is detected by measuring absorbance at 340 nm. Reagents are Research Use Only. |
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Pricing available per request.